other:
Article Title: Generation of an isogenic iPSC line via CRISPR correction of the POMC:W84X mutation for monogenic obesity modeling.
Article Snippet: Antibodies and stains used for immunocytochemistry/flow-cytometry Antibody Dilution Company Cat # and RRID Marker of the hPSC undifferentiated state (FACS) recombinant anti-SSEA-4 VioBlue FACS 1:20 Miltenyi Biotec, 130-098-366AB_2653521 Marker of the hPSC undifferentiated state (FACS/IF) recombinant anti-Oct3/4 APC FACS 1:100IF 1:10 Miltenyi Biotec, 130-123-257AB_2819457 Marker of the hPSC undifferentiated state (FACS/IF) recombinant anti-TRA-1-60 Vio488 FACS 1:700IF 1:100 Miltenyi Biotec, 130-106-872AB_2654228 Marker of the hPSC undifferentiated state (FACS/IF) rabbit anti-Nanog (D73G4) PE FACS 1:100IF 1:50 Cell Signaling, 14955SAB_2798659 Marker of the hPSC undifferentiated state (IF) recombinant anti-SSEA4 PerCpVio700 IF 1:10 Miltenyi Biotec, 130-105-053AB_2653527 Trilineage differentiation (FACS) Sox2 Antibody, anti-human/mouse, FITC, REAfinityTM FACS: 1:250 Miltenyi Biotec, 130-120-721AB_2784458 Trilineage differentiation (FACS) CD140b Antibody, anti-human, APC, REAfinityTM FACS: 1:50 Miltenyi Biotec, 130-105-280AB_2655085 Trilineage differentiation (FACS) CD144 (VE-Cadherin) Antibody, anti-human, FITC, REAfinityTM FACS: 1:50 Miltenyi Biotec,130-100-742AB_2655151 Trilineage differentiation (FACS) CD184 (CXCR4) Antibody, anti-human, APC, REAfinityTM FACS: 1:250 Miltenyi Biotec, 130-120-708AB_2752173 Trilineage differentiation (FACS) Sox17 Antibody, anti-human, Vio B515, REAfinityTM FACS: 1:250 Miltenyi Biotec, 130-111-031AB_2653497 Site-specific nuclease Nuclease information Recombinant HiFi Cas9 Alt-R® S.p.
Isolation:Article Title: Estradiol Reprograms Microglia to Create an Immune-Suppressed Niche Permissive to Breast Cancer Brain Metastasis
Article Snippet: Primary T cells were isolated from spleen and pooled inguinal, brachial, and axillary lymph nodes using the Pan T Cell Isolation Kit II, mouse (Miltenyi Biotec, 130-095-130). .. Microglia were isolated from tumor-bearing brains using CD11b Microglia MicroBeads, human/mouse (Miltenyi Biotec, 130-093-634) and used immediately for co-culture experiments. ..
Chromatin Immunoprecipitation:Article Title: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Antibody details are listed in supplementary table 2 as follows: - Cleaved caspase-3 (Asp175), anti-human, Cell Signaling #9661 (1:200) - Phospho-Histone H2A.X (Ser139), anti-human, clone JBW301, Sigma-Aldrich #05-636 (1:200) - EAAT2 (E-1), anti-human, Santa Cruz #sc-365634 (1:100) - Tubulin !3, clone TUJ1, anti-human, BioLegend #801202 (1:2500) - GFAP, anti-human/mouse/rat, clone REA335, Miltenyi Biotec #130-105-140 (1:50) - Phospho-Histone H2A.X (Ser139), clone CR55T33, anti-mouse/human, Thermo Fisher #12-9865-42 (1:50) - NeuN, anti-human/mouse/rat, clone REA1131, Miltenyi Biotec #130-119-492 (1:50) - Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588], anti-human/mouse/rat, Abcam #ab225261 (1:50) - SOX9 Monoclonal Antibody (GMPR9) Alexa Fluor® 488, anti-human/mouse, Thermo Fisher #53-9765-80 (1:50) - Anti-CD3 epsilon antibody [EPR22667-12], anti-mouse, Abcam #ab231775 (1:50) - CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate), Cell Signaling #81143 (1:50) - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859], anti-mouse, Abcam #ab246711 (1:50) - Galectin-3, anti-human/mouse, APC, Miltenyi Biotech #130-118-649 (1:50) - Donkey anti-rabbit IgG Alexa Fluor 488 Thermo Fisher #A-21206 (1:500) - Donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher #A-31570 (1:500) All antibodies are commercially validated. .. Information can be found using the links below: - Cleaved caspase-3: https://www.cellsignal.com/products/primary-antibodies/cleaved-caspase-3-asp175-antibody/9661 - Phospho-Histone H2A.X: https://www.sigmaaldrich.com/US/en/product/mm/05636 - EAAT2: https://www.scbt.com/p/eaat2-antibody-e-1 - Tubulin !3: https://www.biolegend.com/en-gb/products/purified-anti-tubulin-beta-3-tubb3-antibody-11580 - GFAP: https://www.miltenyibiotec.com/US-en/products/gfap-antibody-anti-human-mouse-rat-reafinityrea335.html#Conjugate=pure:size=100-ug-in-1-mL - Phospho-Histone H2A.X: https://www.thermofisher.com/antibody/product/Phospho-Histone-H2A-X-Ser139-Antibody-cloneCR55T33-Monoclonal/12-9865-42 - NeuN: https://www.miltenyibiotec.com/US-en/products/neun-antibody-anti-human-mouse-rat-reafinityrea1131.html#conjugate=vio-r667:size=600-ul - Alexa Fluor® 647 anti-Iba1: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-647-iba1-antibody-epr16588ab225261 - SOX9: https://www.thermofisher.com/antibody/product/SOX9-Antibody-clone-GMPR9-Monoclonal/53-9765-80 - Anti-CD3 epsilon: https://www.abcam.com/en-us/products/primary-antibodies/cd3-epsilon-antibody-epr22667-12-ab231775 - CD45: https://www.cellsignal.com/products/antibody-conjugates/cd45-d3f8q-rabbit-mab-alexa-fluor-647-conjugate/81143 - Alexa Fluor® 488 anti-GSDMD: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-488-gsdmd-antibodyepr20859-ab246711 - Galectin-3: https://www.miltenyibiotec.com/US-en/products/galectin-3-antibody-anti-human-mouse-reafinityrea1072.html#conjugate=vio-b515:size=100-tests-in-200-ul - Donkey anti-rabbit IgG Alexa Fluor 488: https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-CrossAdsorbed-Secondary-Antibody-Polyclonal/A-21206) - Donkey anti-mouse IgG Alexa Fluor 555: https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-HighlyCross-Adsorbed-Secondary-Antibody-Polyclonal/A-31570 Studies were performed on healthy donor hiPSC clones ND1.3 and clone ND2.2 which have been previously characterized and referenced in the manuscript, and on human primary neurons obtained from Innoprot (Cat# P10151) isolated from healthy brain.
Flow Cytometry:Article Title: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Antibody details are listed in supplementary table 2 as follows: - Cleaved caspase-3 (Asp175), anti-human, Cell Signaling #9661 (1:200) - Phospho-Histone H2A.X (Ser139), anti-human, clone JBW301, Sigma-Aldrich #05-636 (1:200) - EAAT2 (E-1), anti-human, Santa Cruz #sc-365634 (1:100) - Tubulin !3, clone TUJ1, anti-human, BioLegend #801202 (1:2500) - GFAP, anti-human/mouse/rat, clone REA335, Miltenyi Biotec #130-105-140 (1:50) - Phospho-Histone H2A.X (Ser139), clone CR55T33, anti-mouse/human, Thermo Fisher #12-9865-42 (1:50) - NeuN, anti-human/mouse/rat, clone REA1131, Miltenyi Biotec #130-119-492 (1:50) - Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588], anti-human/mouse/rat, Abcam #ab225261 (1:50) - SOX9 Monoclonal Antibody (GMPR9) Alexa Fluor® 488, anti-human/mouse, Thermo Fisher #53-9765-80 (1:50) - Anti-CD3 epsilon antibody [EPR22667-12], anti-mouse, Abcam #ab231775 (1:50) - CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate), Cell Signaling #81143 (1:50) - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859], anti-mouse, Abcam #ab246711 (1:50) - Galectin-3, anti-human/mouse, APC, Miltenyi Biotech #130-118-649 (1:50) - Donkey anti-rabbit IgG Alexa Fluor 488 Thermo Fisher #A-21206 (1:500) - Donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher #A-31570 (1:500) All antibodies are commercially validated. .. Information can be found using the links below: - Cleaved caspase-3: https://www.cellsignal.com/products/primary-antibodies/cleaved-caspase-3-asp175-antibody/9661 - Phospho-Histone H2A.X: https://www.sigmaaldrich.com/US/en/product/mm/05636 - EAAT2: https://www.scbt.com/p/eaat2-antibody-e-1 - Tubulin !3: https://www.biolegend.com/en-gb/products/purified-anti-tubulin-beta-3-tubb3-antibody-11580 - GFAP: https://www.miltenyibiotec.com/US-en/products/gfap-antibody-anti-human-mouse-rat-reafinityrea335.html#Conjugate=pure:size=100-ug-in-1-mL - Phospho-Histone H2A.X: https://www.thermofisher.com/antibody/product/Phospho-Histone-H2A-X-Ser139-Antibody-cloneCR55T33-Monoclonal/12-9865-42 - NeuN: https://www.miltenyibiotec.com/US-en/products/neun-antibody-anti-human-mouse-rat-reafinityrea1131.html#conjugate=vio-r667:size=600-ul - Alexa Fluor® 647 anti-Iba1: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-647-iba1-antibody-epr16588ab225261 - SOX9: https://www.thermofisher.com/antibody/product/SOX9-Antibody-clone-GMPR9-Monoclonal/53-9765-80 - Anti-CD3 epsilon: https://www.abcam.com/en-us/products/primary-antibodies/cd3-epsilon-antibody-epr22667-12-ab231775 - CD45: https://www.cellsignal.com/products/antibody-conjugates/cd45-d3f8q-rabbit-mab-alexa-fluor-647-conjugate/81143 - Alexa Fluor® 488 anti-GSDMD: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-488-gsdmd-antibodyepr20859-ab246711 - Galectin-3: https://www.miltenyibiotec.com/US-en/products/galectin-3-antibody-anti-human-mouse-reafinityrea1072.html#conjugate=vio-b515:size=100-tests-in-200-ul - Donkey anti-rabbit IgG Alexa Fluor 488: https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-CrossAdsorbed-Secondary-Antibody-Polyclonal/A-21206) - Donkey anti-mouse IgG Alexa Fluor 555: https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-HighlyCross-Adsorbed-Secondary-Antibody-Polyclonal/A-31570 Studies were performed on healthy donor hiPSC clones ND1.3 and clone ND2.2 which have been previously characterized and referenced in the manuscript, and on human primary neurons obtained from Innoprot (Cat# P10151) isolated from healthy brain.
Article Title: Unveiling Spontaneous Renal Tubule-Like Structures from Human Adult Renal Progenitor Cell Spheroids Derived from Urine
Article Snippet: .. Spheroids dissociation and Flow Cytometry Analysis For ow cytometry analysis, the dissociation of spheroids into single-cell suspensions was performed using the Embryoid Body Dissociation Kit, human/mouse (Miltenyi Biotec, Bologna, Italy) following manufacturer instructions. ..
Magnetic Resonance Imaging:Article Title: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Antibody details are listed in supplementary table 2 as follows: - Cleaved caspase-3 (Asp175), anti-human, Cell Signaling #9661 (1:200) - Phospho-Histone H2A.X (Ser139), anti-human, clone JBW301, Sigma-Aldrich #05-636 (1:200) - EAAT2 (E-1), anti-human, Santa Cruz #sc-365634 (1:100) - Tubulin !3, clone TUJ1, anti-human, BioLegend #801202 (1:2500) - GFAP, anti-human/mouse/rat, clone REA335, Miltenyi Biotec #130-105-140 (1:50) - Phospho-Histone H2A.X (Ser139), clone CR55T33, anti-mouse/human, Thermo Fisher #12-9865-42 (1:50) - NeuN, anti-human/mouse/rat, clone REA1131, Miltenyi Biotec #130-119-492 (1:50) - Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588], anti-human/mouse/rat, Abcam #ab225261 (1:50) - SOX9 Monoclonal Antibody (GMPR9) Alexa Fluor® 488, anti-human/mouse, Thermo Fisher #53-9765-80 (1:50) - Anti-CD3 epsilon antibody [EPR22667-12], anti-mouse, Abcam #ab231775 (1:50) - CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate), Cell Signaling #81143 (1:50) - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859], anti-mouse, Abcam #ab246711 (1:50) - Galectin-3, anti-human/mouse, APC, Miltenyi Biotech #130-118-649 (1:50) - Donkey anti-rabbit IgG Alexa Fluor 488 Thermo Fisher #A-21206 (1:500) - Donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher #A-31570 (1:500) All antibodies are commercially validated. .. Information can be found using the links below: - Cleaved caspase-3: https://www.cellsignal.com/products/primary-antibodies/cleaved-caspase-3-asp175-antibody/9661 - Phospho-Histone H2A.X: https://www.sigmaaldrich.com/US/en/product/mm/05636 - EAAT2: https://www.scbt.com/p/eaat2-antibody-e-1 - Tubulin !3: https://www.biolegend.com/en-gb/products/purified-anti-tubulin-beta-3-tubb3-antibody-11580 - GFAP: https://www.miltenyibiotec.com/US-en/products/gfap-antibody-anti-human-mouse-rat-reafinityrea335.html#Conjugate=pure:size=100-ug-in-1-mL - Phospho-Histone H2A.X: https://www.thermofisher.com/antibody/product/Phospho-Histone-H2A-X-Ser139-Antibody-cloneCR55T33-Monoclonal/12-9865-42 - NeuN: https://www.miltenyibiotec.com/US-en/products/neun-antibody-anti-human-mouse-rat-reafinityrea1131.html#conjugate=vio-r667:size=600-ul - Alexa Fluor® 647 anti-Iba1: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-647-iba1-antibody-epr16588ab225261 - SOX9: https://www.thermofisher.com/antibody/product/SOX9-Antibody-clone-GMPR9-Monoclonal/53-9765-80 - Anti-CD3 epsilon: https://www.abcam.com/en-us/products/primary-antibodies/cd3-epsilon-antibody-epr22667-12-ab231775 - CD45: https://www.cellsignal.com/products/antibody-conjugates/cd45-d3f8q-rabbit-mab-alexa-fluor-647-conjugate/81143 - Alexa Fluor® 488 anti-GSDMD: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-488-gsdmd-antibodyepr20859-ab246711 - Galectin-3: https://www.miltenyibiotec.com/US-en/products/galectin-3-antibody-anti-human-mouse-reafinityrea1072.html#conjugate=vio-b515:size=100-tests-in-200-ul - Donkey anti-rabbit IgG Alexa Fluor 488: https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-CrossAdsorbed-Secondary-Antibody-Polyclonal/A-21206) - Donkey anti-mouse IgG Alexa Fluor 555: https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-HighlyCross-Adsorbed-Secondary-Antibody-Polyclonal/A-31570 Studies were performed on healthy donor hiPSC clones ND1.3 and clone ND2.2 which have been previously characterized and referenced in the manuscript, and on human primary neurons obtained from Innoprot (Cat# P10151) isolated from healthy brain.
Biomarker Discovery:Article Title: AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain
Article Snippet: .. 3 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Antibody details are listed in supplementary table 2 as follows: - Cleaved caspase-3 (Asp175), anti-human, Cell Signaling #9661 (1:200) - Phospho-Histone H2A.X (Ser139), anti-human, clone JBW301, Sigma-Aldrich #05-636 (1:200) - EAAT2 (E-1), anti-human, Santa Cruz #sc-365634 (1:100) - Tubulin !3, clone TUJ1, anti-human, BioLegend #801202 (1:2500) - GFAP, anti-human/mouse/rat, clone REA335, Miltenyi Biotec #130-105-140 (1:50) - Phospho-Histone H2A.X (Ser139), clone CR55T33, anti-mouse/human, Thermo Fisher #12-9865-42 (1:50) - NeuN, anti-human/mouse/rat, clone REA1131, Miltenyi Biotec #130-119-492 (1:50) - Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588], anti-human/mouse/rat, Abcam #ab225261 (1:50) - SOX9 Monoclonal Antibody (GMPR9) Alexa Fluor® 488, anti-human/mouse, Thermo Fisher #53-9765-80 (1:50) - Anti-CD3 epsilon antibody [EPR22667-12], anti-mouse, Abcam #ab231775 (1:50) - CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 647 Conjugate), Cell Signaling #81143 (1:50) - Alexa Fluor® 488 Anti-GSDMD antibody [EPR20859], anti-mouse, Abcam #ab246711 (1:50) - Galectin-3, anti-human/mouse, APC, Miltenyi Biotech #130-118-649 (1:50) - Donkey anti-rabbit IgG Alexa Fluor 488 Thermo Fisher #A-21206 (1:500) - Donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher #A-31570 (1:500) All antibodies are commercially validated. .. Information can be found using the links below: - Cleaved caspase-3: https://www.cellsignal.com/products/primary-antibodies/cleaved-caspase-3-asp175-antibody/9661 - Phospho-Histone H2A.X: https://www.sigmaaldrich.com/US/en/product/mm/05636 - EAAT2: https://www.scbt.com/p/eaat2-antibody-e-1 - Tubulin !3: https://www.biolegend.com/en-gb/products/purified-anti-tubulin-beta-3-tubb3-antibody-11580 - GFAP: https://www.miltenyibiotec.com/US-en/products/gfap-antibody-anti-human-mouse-rat-reafinityrea335.html#Conjugate=pure:size=100-ug-in-1-mL - Phospho-Histone H2A.X: https://www.thermofisher.com/antibody/product/Phospho-Histone-H2A-X-Ser139-Antibody-cloneCR55T33-Monoclonal/12-9865-42 - NeuN: https://www.miltenyibiotec.com/US-en/products/neun-antibody-anti-human-mouse-rat-reafinityrea1131.html#conjugate=vio-r667:size=600-ul - Alexa Fluor® 647 anti-Iba1: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-647-iba1-antibody-epr16588ab225261 - SOX9: https://www.thermofisher.com/antibody/product/SOX9-Antibody-clone-GMPR9-Monoclonal/53-9765-80 - Anti-CD3 epsilon: https://www.abcam.com/en-us/products/primary-antibodies/cd3-epsilon-antibody-epr22667-12-ab231775 - CD45: https://www.cellsignal.com/products/antibody-conjugates/cd45-d3f8q-rabbit-mab-alexa-fluor-647-conjugate/81143 - Alexa Fluor® 488 anti-GSDMD: https://www.abcam.com/en-us/products/primary-antibodies/alexa-fluor-488-gsdmd-antibodyepr20859-ab246711 - Galectin-3: https://www.miltenyibiotec.com/US-en/products/galectin-3-antibody-anti-human-mouse-reafinityrea1072.html#conjugate=vio-b515:size=100-tests-in-200-ul - Donkey anti-rabbit IgG Alexa Fluor 488: https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-CrossAdsorbed-Secondary-Antibody-Polyclonal/A-21206) - Donkey anti-mouse IgG Alexa Fluor 555: https://www.thermofisher.com/antibody/product/Donkey-anti-Mouse-IgG-H-L-HighlyCross-Adsorbed-Secondary-Antibody-Polyclonal/A-31570 Studies were performed on healthy donor hiPSC clones ND1.3 and clone ND2.2 which have been previously characterized and referenced in the manuscript, and on human primary neurons obtained from Innoprot (Cat# P10151) isolated from healthy brain.
Cytometry:Article Title: Unveiling Spontaneous Renal Tubule-Like Structures from Human Adult Renal Progenitor Cell Spheroids Derived from Urine
Article Snippet: .. Spheroids dissociation and Flow Cytometry Analysis For ow cytometry analysis, the dissociation of spheroids into single-cell suspensions was performed using the Embryoid Body Dissociation Kit, human/mouse (Miltenyi Biotec, Bologna, Italy) following manufacturer instructions. ..
Single Cell:Article Title: Unveiling Spontaneous Renal Tubule-Like Structures from Human Adult Renal Progenitor Cell Spheroids Derived from Urine
Article Snippet: .. Spheroids dissociation and Flow Cytometry Analysis For ow cytometry analysis, the dissociation of spheroids into single-cell suspensions was performed using the Embryoid Body Dissociation Kit, human/mouse (Miltenyi Biotec, Bologna, Italy) following manufacturer instructions. ..
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